Journal: Frontiers in Plant Science

Article Title: Integrated Gene Co-expression Analysis and Metabolites Profiling Highlight the Important Role of ZmHIR3 in Maize Resistance to Gibberella Stalk Rot

doi: 10.3389/fpls.2021.664733

Figure Lengend Snippet: Identification and GSR phenotypic analysis of zmhir3 mutant in response to F. graminearum infection. (A) Identification of zmhir3 EMS mutant by resequencing. The top schematic diagram represents the SNP mutation in ZmHIR3 gene. The red arrow shows the mutation of G to A in the end of second intron of gene. The bottom indicates the mutation of SNP G to A by resequencing. (B) Subcellular localization of ZmHIR3 on the plasma membrane. BF, bright field. (C) Seedling GSR phenotypes of zmhir3 mutant versus wild-type B73. The phenotype was recorded at 3 dpi. (D) Quantification of seedling GSR disease index (DSI) of zmhir3 mutant vs. wild-type B73. (E) Field GSR phenotypes of zmhir3 mutant vs. wild-type B73 recorded at 15 dpi. (F) GSR disease areas on the stems of zmhir3 mutant vs. wild-type B73 adult plants in the field condition. ∗∗ Statistically significant ( p < 0.01) between mock and inoculation treatments analyzed by t -test. (G) Trypan blue staining of cell death on the stems of zmhir3 mutant vs. B73 seedlings at different time points upon infection with F. graminearum . The scale on the picture is 50 μm. hpi, hours postinfection.

Article Snippet: Paired-end RNA sequence reads of 150 bases were generated, and all clean reads from sequencing were aligned to maize inbred B73 reference genome (RefGen_V4) and the reference gene model dataset of F. graminearum (FGS 5b) using hisat2.

Techniques: Mutagenesis, Infection, Clinical Proteomics, Membrane, Staining